| Titre : |
Tobacco consumption and benzo(a)pyrene-diol- epoxide–DNA adducts in spermatozoa: in smokers, swim-up procedure selects spermatozoa with decreased DNA damage |
| Type de document : |
document électronique |
| Auteurs : |
Jeanne Perrin, Auteur ; Virginie Tassistro, Auteur ; Marion Mandon, Auteur |
| Editeur : |
New-York [États-Unis] : Elsevier Science |
| Année de publication : |
2011 |
| Collection : |
Fertility and Sterility, ISSN 1556-5653 num. Vol. 95, No. 6 |
| Importance : |
p.2013-2017 |
| Langues : |
Anglais (eng) |
| Catégories : |
[DIVERS] personne:par métier:professionnel de la santé
[TABAC] sevrage tabagique:méthode de sevrage:méthode individuelle:approche pharmacologique
[TABAC] tabagisme:effet du tabac:altération de la fonction sexuelle:effet sur la fertilité
[TABAC] tabagisme:risque:facteur associé:génétique
|
| Index. décimale : |
TA 3.2.2.7 Grossesse et fœtus |
| Résumé : |
Objective: To analyze the distribution of benzo(a)pyrene-diol-epoxide (BPDE)–DNA adducts in spermatozoa selected and nonselected by a swim-up procedure with relation to smoking habits.
Design: Comparative study.
Setting: Public university and public university hospital.
Patient(s): Seventy-nine men (37 smokers and 42 nonsmokers) who visited an infertility clinic for diagnostic.
Intervention(s) :Tobacco and environmental exposure assessment, semen sample analysis, swim-up procedure, BPDE-DNA adduct immunolabeling.
Main Outcome Measure(s):
BPDE-DNA adduct quantification in selected (SEL-SPZ) and nonselected (NONSEL-SPZ) spermatozoa. Data were normalized by using a normalized fluorescence value (NFV).
Result(s) :
The mean NFV (±SD) in SEL-SPZ was significantly higher in smokers than in nonsmokers (18.9 ± 11.5 vs. 10.5 ± 10.4, respectively). Within smokers, a paired analysis (SEL-SPZ and NONSEL-SPZ) showed that NFV was significantly lower in SEL-SPZ than in NONSEL-SPZ (20.0 ± 11.3 vs. 31.5 ± 16.0, respectively). Conversely, within nonsmokers, the mean NFV was higher in SEL-SPZ than in NONSEL-SPZ (10.3 ± 10.6 vs 4.3 ± 7.1, respectively).
Conclusion(s) :
Tobacco consumption is associated with BPDE-DNA adducts in spermatozoa. In smokers, semen processing by swim-up recovers potentially fertilizing spermatozoa that show a significantly lower amount of BPDE-DNA adducts compared with NONSEL-SPZ. Further study is needed to improve the spermatozoa selection in smoking patients requiring assisted reproductive technologies. |
| En ligne : |
https://doi.org/10.1016/j.fertnstert.2011.02.021 |
| Format de la ressource électronique : |
Article en ligne |
| Permalink : |
https://biblio.fares.be/opac_css/index.php?lvl=notice_display&id=10108 |
Tobacco consumption and benzo(a)pyrene-diol- epoxide–DNA adducts in spermatozoa: in smokers, swim-up procedure selects spermatozoa with decreased DNA damage (2011)
