| Titre : |
Single-cell RNA sequencing reveals heterogeneity of ALI model and epithelial cell alterations after exposure to electronic cigarette vapor |
| Type de document : |
document électronique |
| Auteurs : |
Meng-yun Cai, Auteur ; Xiaofan Mao, Auteur ; Beiying Zhang, Auteur |
| Editeur : |
Research Square |
| Année de publication : |
2023 |
| Importance : |
23 p. |
| Présentation : |
graph.; ill. |
| Langues : |
Anglais (eng) |
| Catégories : |
[TABAC] chimie du tabac:constituant:additif:agent de saveur
[TABAC] chimie du tabac:constituant:alcaloïde:nicotine
[TABAC] chimie du tabac:tabac fumé:cigarette:cigarette électronique
[TABAC] étude
[TABAC] tabagisme:risque
[TABAC] tabagisme:tabagisme actif
|
| Index. décimale : |
TA 3.2.2.4 Pathologies respiratoires (sauf 3.2.2.1, 3.2.2.2, 3.2.2.3) |
| Résumé : |
Background
Electronic cigarettes (e-cigarettes) have been advertised as a healthier alternative to traditional cigarettes; however, their exact effects on the bronchial epithelium are poorly understood. Air-liquid interface (ALI) culture allows human primary bronchial epithelial cells to differentiate into bronchial epithelium (ALI-HBE), providing an in vitro model that simulates the biological characteristics of normal bronchial epithelium.
Methods
Single-cell RNA sequencing of ALI-HBE was used to reveal previously unrecognized transcriptional heterogeneity within the human bronchial epithelium and cell type–specific responses to acute exposure to e-cigarette vapor (e-vapor) containing distinct components (nicotine and/or flavoring).
Results
Acute exposure to e-vapor containing nicotine affected gene expression related to secretory function and basal-to-secretory transformation. In addition, acute exposure to e-vapor containing flavoring might promote susceptibility to virus infection and activate epithelial-to-mesenchymal transition.
Conclusion
The ALI-HBE model recapitulates the heterogeneity and transcriptional characteristics of human bronchial epithelium. Single-cell sequencing data provided high-resolution insights into e-vapor–induced remodeling of bronchial epithelium. The data also indicate factors on bronchial epithelial cells that may promote SARS-CoV-2 infection and suggest therapeutic targets for restoring normal bronchial epithelium function after e-cigarette use. |
| En ligne : |
https://doi.org/10.21203/rs.3.rs-3708689/v1 |
| Format de la ressource électronique : |
Article en ligne |
| Permalink : |
https://biblio.fares.be/opac_css/index.php?lvl=notice_display&id=10580 |
Single-cell RNA sequencing reveals heterogeneity of ALI model and epithelial cell alterations after exposure to electronic cigarette vapor (2023)
