| Titre : |
Electronic cigarettes induce DNA strand breaks and cell death independently of nicotine in cell lines |
| Type de document : |
document électronique |
| Auteurs : |
Vicky Yu, Auteur ; Mehran Rahimy, Auteur ; Avinaash Korrapati, Auteur |
| Editeur : |
Paris [France] : Elsevier |
| Année de publication : |
2016 |
| Collection : |
Oral Oncology, ISSN 1368-8375 |
| Importance : |
p. 58-65 |
| Présentation : |
graph., ill., tab. |
| Langues : |
Anglais (eng) |
| Catégories : |
[TABAC] chimie du tabac:constituant:alcaloïde:nicotine
[TABAC] chimie du tabac:tabac fumé:cigarette:cigarette électronique
[TABAC] tabagisme:effet du tabac:toxicité
[TABAC] tabagisme:risque:facteur associé:génétique
|
| Index. décimale : |
TA 3.2.2.6 Effets métaboliques et biologiques |
| Résumé : |
Objectives: Evaluate the cytotoxicity and genotoxicity of short- and long-term e-cigarette vapor exposure on a panel of normal epithelial and head and neck squamous cell carcinoma (HNSCC) cell lines.
Materials and methods: HaCaT, UMSCC10B, and HN30 were treated with nicotine-containing and
nicotine-free vapor extract from two popular e-cigarette brands for periods ranging from 48 h to 8 weeks.
Cytotoxicity was assessed using Annexin V flow cytometric analysis, trypan blue exclusion, and clono?genic assays. Genotoxicity in the form of DNA strand breaks was quantified using the neutral comet assay and c-H2AX immunostaining.
Results: E-cigarette-exposed cells showed significantly reduced cell viability and clonogenic survival, along with increased rates of apoptosis and necrosis, regardless of e-cigarette vapor nicotine content. They also exhibited significantly increased comet tail length and accumulation of c-H2AX foci, demon?strating increased DNA strand breaks.
Conclusion: E-cigarette vapor, both with and without nicotine, is cytotoxic to epithelial cell lines and is a DNA strand break-inducing agent. Further assessment of the potential carcinogenic effects of e-cigarette vapor is urgently needed. |
| En ligne : |
https://doi.org/10.1016%2Fj.oraloncology.2015.10.018 |
| Format de la ressource électronique : |
PDF |
| Permalink : |
https://biblio.fares.be/opac_css/index.php?lvl=notice_display&id=9562 |
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