Vaping-dependent pulmonary inflammation is Ca2+ mediated and potentially sex specific / Jeffrey G Shipman (2024)  

Public Titre : Vaping-dependent pulmonary inflammation is Ca2+ mediated and potentially sex specific Type de document : document électronique Auteurs : Jeffrey G Shipman, Auteur ; Rob U. Onyenwoke, Auteur ; Vijay Sivaraman, Auteur Editeur : Bâle [Suisse] : MDPI AG (Molecular Diversity Preservation International) Année de publication : 2024 Collection : International journal of molecular sciences num. 25 Importance : 12 p. Présentation : ill., tab., graph. Langues : Anglais (eng) Catégories : [TABAC] CANDIDATS:e-cigarette [TABAC] tabagisme:pathologie:pathologie respiratoire:coeur pulmonaire Mots-clés : expérimentation animale Index. décimale : TA 1.1.1 Cigarettes (« normales », électroniques, aromatisées,…) Résumé : Here we use the SCIREQ InExpose system to simulate a biologically relevant vaping model in mice to investigate the role of calcium signaling in vape-dependent pulmonary disease as well as to investigate if there is a gender-based difference of disease. Male and female mice were vaped with JUUL Menthol (3% nicotine) using the SCIREQ InExpose system for 2 weeks. Additionally, 2-APB, a known calcium signaling inhibitor, was administered as a prophylactic for lung disease and damage caused by vaping. After 2 weeks, mice were exposed to lipopolysaccharide (LPS) to mimic a bacterial infection. Post-infection (24 h), mice were sacrificed, and bronchoalveolar lavage fluid (BALF) and lungs were taken. Vaping primed the lungs for worsened disease burden after microbial challenge (LPS) for both males and females, though females presented increased neutrophilia and inflammatory cytokines post-vape compared to males, which was assessed by flow cytometry, and cytokine and histopathological analysis. This increased inflammatory burden was controlled by calcium signaling inhibition, suggesting that calcium dysregulation may play a role in lung injury caused by vaping in a gender-dependent manner. En ligne : https://doi.org/ 10.3390/ijms25031785 Format de la ressource électronique : Article en ligne Permalink : https://biblio.fares.be/opac_css/index.php?lvl=notice_display&id=10309

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